Immunization

Our extensive suite of immunization technologies can be used across multiple (transgenic) species, including mice, rats, rabbits, chickens, and camelids. Based on target analysis, lead antibody requirements and our experience, we will propose the most favorable immunization approach.

AVS Bio offers multiple immunization options, with flexibility in injection methods or routes and types of immunogens. To promote a diverse antibody response, we enable clients to alternate immunization methods and routes as well as immunogen types.

Resources:

• Service Sheet Antibody Discovery
• Poster "Harnessing diversity-focused mouse B cell discovery and early stage, high-throughput characterization"
• Poster "Advancing VHH discovery leveraging a high throughput B cell-based platform"

Immunogens

DNA

Our proprietary genetic immunizations approach enables the generation of a specific antibody response against structurally complex and conformation-sensitive targets, such as GPCRs and ion channels. Using optimized vectors developed in house, we achieve high in vivo expression of targets with appropriate post-translational modifications.

mRNA

mRNA-based immunizations do not require transcription, enabling faster and more straightforward transient target expression in vivo, which is advantageous for certain types of targets. Combined with a rapid and flexible manufacturing process, mRNA immunization is a powerful approach.

Our mRNA-based immunizations do not require transcription, enabling faster and more straightforward transient target expression in vivo, which is advantageous for certain types of targets. Combined with a rapid and flexible manufacturing process, mRNA immunization is a powerful approach.

(Syngeneic) Cell Line

Cell lines (over)expressing targets of interest can be used as immunogens, which allows for presentation of targets in a more native context to the immune system. AVS Bio can aid in selection of suitable endogenous target-expressing cell lines or can generate target overexpressing cell lines, ideally in syngeneic cell lines.

Recombinant Protein

For antibody discovery projects with conformationally less challenging proteins, immunization with recombinant protein is the method of choice—a fast, cost-effective, and efficient way to elicit strong antibody responses. AVS Bio provides custom recombinant protein production, optimized for use in immunizations, ensuring that your discovery project can move forward without delay.

VLP / Liposome

In cases where stable expression of the protein of interest on cells is challenging, we can use virus-like particles (VLPs), liposomes or other membrane-mimetics. These delivery systems present antigens in a highly immunogenic, multivalent format, making them indispensable for immunization against challenging or low-abundance targets. By mimicking natural viral or membrane structures, VLPs and liposomes enhance antigen presentation and improve the likelihood of generating strong, specific immune responses.

Peptide

If analysis of your target and lead antibody requirements identifies peptides as the immunogen of choice, AVS Bio’s computational scientists can assist you with peptide design based on in-depth modeling and immunogenic-potential determinations. We can also advise on the most convenient modifications for carrier conjugation and use in downstream selections and screenings.