Complement Factor B, Human, Man5-Glcnac2 N-Linked Glycans

Complement Factor B, Human, Man5-Glcnac2 N-Linked Glycans
Complement factor B is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. The cDNA coding for Factor B was cloned and expressed in HEK293EBNA1 cells that are deficient in an enzyme that is involved in N-linked glycosylation of glycoproteins. As a result, recombinant factor B produced from this cell line contains N-linked glycans of the short and simple man5-GlcNAc2 type. Therefor, this factor B is extremely suitable for protein crystallization experiments. Factor B is purified to homogeneity.

Production platform: This product has been produced in HEK293 cells using the rPEx® recombinant protein production platform.