Mouse anti-human Thrombospondin

Mouse anti-Thrombospondin
Antibody against thrombospondin 1 (TSP-1). TSP-1 was first isolated from platelets (~120 kDa monomer) that had been stimulated with thrombin, and so was designated 'thrombin-sensitive protein'. Since its first recognition, functions for TSP-1 have been found in multiple biological processes including angiogenesis, apoptosis, activation of TGF-beta and immune regulation. TSP-1 is an antiangiogenic, inhibiting the proliferation and migration of endothelial cells by interactions with CD36 expressed on their surface of these cells, leading to the expression of FAS ligand, which activates its specific, ubiquitous receptor, Fas. This leads to the activation of caspases and apoptosis of the cell.
This antibody was generated against activated platelets and recognized a major protein of 33 Kd and a minor 28-Kd protein, both under nonreduced and reduced conditions. It binds to the N-terminal proteolytic fragment GMP-33 of human thrombospondin and reacts only with reduced thrombospondin. Plasmin and trypsin digestion of thrombospondin yielded fragments of approximately the same size as GMP 33 that react with this antibody after reduction.